Background: Apart from clinical examination and laboratory examination to demonstrate Lepra Bacilli from slit skin smear examination of clinically suspected patients with microbiological grading of cases & an attempt is taken to develop an artificial culture media for growth of Lepra Bacilli by inclusion of their requirement present in natural habitat. Methods: This is a prospective study involving 100 clinically suspected Leprosy patients who were attended OPD in the Department of Dermatology in a Tertiary care Hospital, Kolkata, India from April 2013 to October 2014. Slit skin smear examination and microbiological grading of the cases were done followed by in-vitro culture from multi-bacillary cases were done in Dept. of Microbiology of said hospital. Statistical analysis was done by using pie charts and bar charts. Results: The study reveals 58% of pauci-bacillary cases and 42% of multi-bacillary cases. Pauci-bacillary cases are more in number in newly diagnosed cases as well as old treated cases. Incidence and prevalence of leprosy are more in males than in females. Pauci-bacillary cases (59%) are more in male and female both than multi-bacillary cases (41%). Problem is more in rural area than urban. In my study, among 80 confirmed leprosy cases 2 pauci-bacillary cases were considered as relapsed cases as both of them were found to be persistently slit skin smear positive. In both cases no decline in morphological index were detected. Conclusions: Leprosy is associated with lower socioeconomic status predominantly in rural areas, close contact with an index case especially of lepromatous leprosy, lack of BCG vaccination and male preponderance. So, socioeconomic improvements in nutrition, housing and sanitation are key factors in altering immune susceptibility of leprosy transmission. An attempt was taken regarding in-vitro cultivation and considering growth characteristics in natural habitat, at the end of my work I have isolated a Corynebacterium species (Corynebacterium ureicelerivorans) in my developed cultural media.