Impaired vision due to cataract is the leading cause of blindness worldwide. The pathogenesis of senile cataract is considered to be multifactorial in nature. The metabolism of Homocysteine is done with the help of Vitamin B12 and Folate, which converts the same to methionine. Therefore, it can be deduced that Vitamin B12 and Folate act as strong preventive factor for cataract. The present study was conducted with collaboration of Department of Biochemistry at AIIMS, Patna and Department of Ophthalmology, DMCH, Dumka. This study included 50 cataract patients and 20 age matched control subjects. A statistically significant elevation in the level of Homocysteine was observed (p<0.001) in cataract patients as compared with that of control subjects. The plasma levels of folate were observed to be lesser in cataract patients with statistical significance (p<0.001). The random blood glucose levels of patients in the present study were within the normal range, suggesting that hyper-homocysteinemia along with decreased levels of Folate and Vit. B12 can play a significant role in development of cataract in addition to absence of hyper-glycemia or any other virtual metabolic and nutritional disorder as was evident by total protein and albumin levels.