Aim: The present study aimed to assess any gender predilections along with the side (right or left) predominance. Material and method: The study was conducted in the Dental section of Sub district Hospital Nowshera among 384 patients (220 males & 164 females) with age between 18-60 years. 768 mandibular first molars present in these subjects were evaluated using periapical radiographs one in orthoradial and the other taken either 30 degree mesially or distally. The radiographs were separately inspected by two end odontists after placing them over a viewing box under optimal conditions using magnifying glasses (2X). The criteria used to indicate the presence of RE were clear distinction of an extra root, indicated by the crossing of translucent lines defining the pulp space and periodontal ligaments, originating in the upper half of the distal root. Results: Four point ninety five percent of the subjected were having Radix Entomolaris (RE). Radix Entomolaris (RE) was revealed more in females as compared to males. Equal distribution of RE was found among in relation to its side. Conclusion: Clinicians must be well aware of RE and should have knowledge about their prevalence and take every possible measure using the advanced diagnostic and imaging modalities to avoid any endodontic failure leading to high rate of treatment success and patient satisfaction.