Introduction: A new point-of-care testing device recently introduced on the Ghanaian medical market is the Mission Cholesterol Meter. Aim of the study: The overall aim of the study was to evaluate the analytical performance of the Mission Cholesterol meter using NCEP performance criteria. Methods: Same-day testing of fresh normolipidaemic and dyslipidaemic serum samples were done using a certified laboratory method and the Mission Cholesterol meter in 10 replicates. Results: The results showed that the analytical imprecision for all the lipid parameters measured were in the allowable limits. However, the analytical biases for triglycerides and total cholesterol were out of the allowable limits. Conclusion: The Mission Cholesterol meter meets most of the limits of analytical acceptability by the NCEP guidelines. Further validation using a bigger sample size, in terms of its performance to properly classify individuals in different lipid ranges and potential metabolic interference factors is recommended