Aim of study: To assess the effect of fixed orthodontic appliances usage on the level of nickel and chromium in gingival crevicular fluid. Materials and methods: For the study, 18 orthodontics patients were screened including 10 females and 8 males. For the purpose of Gingival Crevicular Fluid (GCF) collection, four different regions were arbitrarily chosen for each patient. This was done to omit any bias associated to site of collection of GCF. Finally, the GCF collection was completes using cellulose acetic acid derivation spongy strip with 45-mm micropores. The strips were introduced very softly in the gingival sulcus for 60 seconds. Statistical Analysis and Results: Statistical analysis was done using statistical software ‘Statistical Package for the Social Sciences (SPSS)’. The recorded data was subjected to suitable statistical tests to obtain p values and mean. P ≤ 0.05 was considered as statistically significant. Statistical analysis & results further revealed that the mean age of the patients was 22.9+6.3 years, ranging from 15-28 years. Authors also noted that there was considerable raise in the mean level of nickel from 1^st visit to 3^rd visit and from 2^nd visit to 3^rd visit. Conclusion: Authors concluded that levels of nickel and chromium in the GCF of studied patients were rising significantly as the duration for treatment progresses.