Introduction: The study investigated the level of knowledge of Peptic Ulcer Disease among final-year Physician Assistantship and Nursing students at Garden City University College. Methods: A structured questionnaire on thematic areas of the condition, including etiology, symptoms, predisposing factors, differential diagnosis, complications, diagnosis, pathophysiological differentiation and treatment was used to assess the participants’ knowledge. Results: The overall mean knowledge was 64.0% for Physician Assistantship students and 53.5% among the Nursing students. The proportion of students with ‘‘Good or Adequate’’ knowledge was 37.5% in the Physician Assistantship class and 9.0% in the Nursing class. Majority of the students had average or moderate knowledge on the specific parameters in the questionnaire. The only exception was in the area of pathophysiological differentiation between gastric ulcer and duodenal ulcer. Conclusion: There is average knowledge of PUD among the students, hence a need for improvement in curricular and implementation to compensate for this knowledge gap.