Neonatal septicemia is an important cause of Neonatal morbidity and mortality. it is one of the indicator for measuring the health status of a nation. According to World Health Organization (WHO) estimates there are 5 million neonatal deaths a year with 98% occurring in developing countries. The incidence of neonatal sepsis varies from 11-24.5 /1000 live births in India. Neonatal deaths account for over a one-third of 1 the global burden of child mortality. Neonatal sepsis can be divided into two main classes depending on the onset of symptoms namely Early-onset sepsis, which usually presents within the first 72 hours of birth and Late2,3 onset sepsis, which usually presents 72 hours after birth. Hence based on above findings the present study was planned for Evaluation of Prevalence of Neonatal Sepsis Relating to Microbial Profile and Sensitivity Pattern in Cases from Darbhanga medical College and Hospital, Darbhanga, Bihar. The present study was planned in Department of Paediatrics, Darbhanga medical College and Hospital, Darbhanga, Bihar, India. The study was conducted from May 2019 to Nov 2019. In the present study 20 cases of neonates suffered from the septicemia were enrolled and evaluated in the present study. The data generated from the present study concludes that Gram negative bacteria were more commonly the cause of septicemia in neonates, and Klebsiella pneumoniae was the predominant pathogen. The positive blood culture with antibiotic sensitivity of the isolated organism is the best guide to antimicrobial therapy, as resistance to antibiotics is a worldwide problem that causes ineffectiveness of empirical treatment. More so, strict infection control practices combined with judicious use of antibiotic therapy are the main solutions to this problem.