Aims and Objectives: This study is aimed to establish normal reference range of thyroid gland volume in North Indian subjects using ultrasound. Materials and Methods:A total of 100 healthy subjects, 76 (76%) females and 24 (24%) males, were prospectively studied from oct - dec 2019.Samsung H60 with 7-12 MHz linear probe was used in the study. Thyroid volume was estimated using ellipsoid formula with correction factor of 0.524. Results: The overall mean volume ± SD volume of the thyroid gland for both lobes in all the patients studied was 5.95 ± 3.23 ml. The mean volume for both lobes in males and females were 7.82 ± 4.76 ml and 5.36 ± 2.31 ml respectively. The mean volume of right and left lobes of thyroid gland in both males and females were 3.28 ± 1.79 ml and 2.66 ± 1.53 ml respectively. Discussion: Diagnosis of goiter/thyromegaly is based on volume measurement. Volume measurement of the thyroid gland is easy to obtain by ultrasound. This study has shown that the right thyroid lobe volume is greater than the left with significant statistical difference. Conclusion: This study provides normative reference values of thyroid volume which can be used in diagnosis and follow-up of patients with thyroid diseases.