Introduction: The surgical treatment of neoplastic and traumatic lesion and grafting of liver by respective measures has increased in frequency and magnitude during recent years. With recent developments in surgery for effective grafting of liver. The morphological mensurative data of the portal venous system is important, as regards portal hypertension and surgical aspects related to portocaval anatomosis. Aim & Objectives: The aim of present study was to identify anatomical variations of portal vein, which might help for surgeons in resection and grafting of liver during surgeries. Materials & Methods: The study was conducted in the Department of Anatomy Index Medical College & Research center Indore M. P. on sixty adult human livers by dissection method. The liver parenchyma was removed with the help of forceps & ramification patterns of portal veins were studied. Observations & Results: The Portal vein had normal standard pattern in 30% cases and the rest 70% showed variations in one or the other form. The splenic vein was variant in 76.6% cases. The superior mesenteric vein showed variation in 73.3% cases. The mode of termination of the inferior mesenteric vein was variable in 60% of cases. The intrahepatic ramification pattern of portal vein was observed in all the segments of liver. The findings were tabulated according to the measurements taken, analyzed and were statically evaluated using SPSS -16.0 version.