Neonatal Sepsis is a clinical syndrome resulting from pathophysiologic effects in the 1st month of life. Hematological scoring system (HSS) is the scoring system that includes 7 hematological parameter into account and assigns a score which helps in early diagnosis and treatment of sepsis. Aims and Objectives. To assess the importance of hematological parameters in the early diagnosis of neonatal sepsis, compare the variables with blood culture and assess the most sensitive and specific variables in diagnosing neonatal sepsis. Material and method. This is a prospective study done on 105 neonates admitted in NICU of CSSH Meerut from Dec 2020 to Aug 2022 with suspicion of sepsis. Results. HSS was seen to have high sensitivity (>98%) when compared with blood culture, it identified sepsis in > 95% of neonates. Among all parameters of HSS, immature PMN count show highest sensitivity (93%) followed by IT PMN ratio (91%) which also showed high specificity (94%) and positive predictive value. Platelet count was found to have lowest sensitivity but high specificity and high positive predictive value. Conclusion: Hematological scoring system can be considered as a simple, reliable, quick and cost effective test for the early diagnosis of neonatal sepsis. This test helps treating physician to diagnose the neonatal sepsis as early as possible and start the appropriate therapy to prevent sepsis related mortality and morbidity.