Background: In microscopic assessment of mineralized tissue, decalcification is an important step during tissue processing. In this present study conventional method of decalcification was compared with microwave assisted method of decalcification using a rat bone in 5% HCl. Aims: To compare conventional decalcification with microwave assisted decalcification in a bone tissue. To decalcify bone tissue using microwave method of decalcification. To decalcify bone tissue using conventional method of decalcification. Methods: A 2mm sized harvested hindlimbs and spine from a laboratory rat was subjected to both conventional and microwave assisted method decalcification using 5% HCl. The endpoint of decalcification was checked using the physical method. The specimens were then subjected to processing, sectioning and staining with haematoxylin and eosin. The stained sections were observed under a digital microscope. Results: The results in the present study confirmed the fact that the microwave assisted method of decalcification was faster than that of the conventional method and produced a good histological picture and the microwave method took fifty minutes whilst the conventional method took twenty-four hours to decalcify. Conclusion: We concluded that the microwave assisted method of decalcification was faster and better histological image were achieved when compared with the conventional method of decalcification.