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VOL. 11, ISSUE 5 (2025)
To differentiate primary and secondary dengue cases by IgG ELISA method in a tertiary care hospital at Rajasthan
Authors
Jyoti Rana, Dr. Yogendra K Tiwari
Abstract
Dengue remains a major arboviral infection in
India with periodic outbreaks and considerable clinical burden. In 2017; NVBDCP
reported 157220 positive dengue cases with 250 deaths in India. “In most cases,
primary dengue infection runs a mild or self-limiting course. Severe
manifestations such as dengue hemorrhagic fever and dengue shock syndrome are
more commonly linked to immune responses triggered during secondary infections
due to serotype cross-reactivity. Therefore, early recognition of secondary dengue
infection at the initial phase of illness carries significant clinical value.
“Thus, distinguishing primary from secondary dengue infections at an early
stage is clinically important, as it carries significant prognostic value. The
current study is a hospital based prospective analytical evaluation and was
aimed to discriminate secondary from primary dengue virus infection in
clinically suspected dengue cases presenting with fever and thrombocytopenia.
Patients of all age groups attending SRG Hospital on outpatient and inpatient
basis with clinically suspected dengue fever were included in the study. The
samples were tested in the VRDL of the Microbiology Department for dengue NS1
antigen and IgM antibodies, positive for both were further subjected to IgG
antibodies. IgM /IgG ratio was used to differentiate primary and secondary
dengue infections. Dengue infection was categorized based on WHO guidelines. A
total of 652 samples were tested from November 2021 to April 2024; out of 652
samples tested 215(32.97%) were positive for dengue NS1 and IgM. Of the 215
dengue NS1 and IgM positive cases, 75 (34.88%) were having secondary dengue
infection, whereas 140 (65.11%) were having primary infection. Early detection
of secondary infection helps the clinician in anticipating dengue related
complications with appropriate therapeutic intervention, thereby reducing
further complications and mortality.
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Pages:16-19
How to cite this article:
Jyoti Rana, Dr. Yogendra K Tiwari "To differentiate primary and secondary dengue cases by IgG ELISA method in a tertiary care hospital at Rajasthan". International Journal of Medical and Health Research, Vol 11, Issue 5, 2025, Pages 16-19
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